"GLO-1" Related Products

Glyoxalase I Assay Kit

Cat.No.: Kit-0371
Product Overview: Glyoxalase I Assay Kit is a quantitative colorimetric determination of glyoxalase I at 240 nm.
Description: GLYOXALASE I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S- lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methylglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans and human cancer.Simple, direct and automation-ready procedures for measuring GLO-1 activity in biological samples are highly desirable in research and drug discovery. Glyoxalase I Assay Kit provides a sensitive and convenient method for GLO-1 activity determination. The method involves monitoring the increase in the product of the GLO-1 reaction, S- lactoylglutathione, by measuring the change in absorbance at 240 nm.
Applications: Direct Assays: GLO-1 activity in enzyme preparations or biological samples.Drug Discovery/Pharmacology: effects of drugs on GLO-1 activity.
Usage: For research use only (RUO)
Storage: Store the plate at room temperature and other components at -20°C. Shelf life: 12 months after receipt.
Kit Components: Assay Buffer (pH 6.6) 20 mL96 well UV Titer Plate 1 PlateSubstrate 1 mLCosubstrate 1 mL
Detection method: Colorimetric
Compatible Sample Types: Biological Sample
Features & Benefits: Sensitive and accurate. Detection limit 4 U/L GLO-1 activity.Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate. Can be readily automated as a high-throughput assay for thousands of samples per day

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.