Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Epigenetic Kits > HDAC3 Inhibitor Screening Kit

HDAC3 Inhibitor Screening Kit

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Cat.No.:
Kit-0432
Product Overview:
HDAC3 Inhibitor Screening Kit is used for screening HDAC3 inhibitor.
Description:
Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In HDAC3 Inhibitor Screening Kit, HDAC-3 and developer will deacetylate and cleave the substrate [Arg-His-Lys-Lys(Ac)-AFC] to release the AFC molecule, which can be detected by fluorometric method (Ex/Em = 380/500 nm). In presence of a HDAC-3 inhibitor, the cleavage will be inhibited. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-3 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare the efficacy of the test inhibitors.
Usage:
For research use only (RUO)
Storage:
Store the kit at -20°C, protect from light. Store HDAC-3 enzyme at -80°C. Allow Assay Buffer to warm to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.
Kit Components:
HDAC3 Assay Buffer. Cap color: WM. 25 mlHDAC3 Substrate. Cap color: red. 200 µlHDAC-3 Enzyme. Cap color: green. 200 µlDeveloper. Cap color: orange. 1 mlTrichostatin A. Cap color: blue. 50 µl
Detection method:
Fluorometric


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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