The Kinetic HDAC3 Assay Kit is a complete assay system designed to measure histone deacetylase 3 (HDAC3) activity kinetically for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC3 activity measurements. In addition, the kit includes purified HDAC3/NCOR2 enzyme complex and a potent HDAC inhibitor, SAHA, for use as a positive control. The Kinetic HDAC3 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only one simple step for setting up the HDAC3 reaction on a microtiter plate is needed and then the HDAC3 activity level can be measured kinetically using a fluorescence reader.
Great for studying enzyme kinetics and screening small molecular inhibitors of HDAC3 for drug discovery and HTS applications.
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
HDAC3/NCOR2 human recombinant enzyme: 2 µg; -80°C Fluorogenic HDAC substrate (5 mM): 50 µl; -80°C 10x HDAC Developer: 1 ml; -80°C Developer Dilution Buffer: 10 ml; -20°C SAHA (1 mM): 20 µl; -20°C HDAC Assay Buffer: 20 ml; -20°C BSA (1mg/ml): 1 ml; -20°C black, low binding NUNC black microtiter plate: 1 plate; Room temp.