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HEK 293 RELA Nuclear Translocation Assay Kit

Cat.No.: Kit-1652
Product Overview: Nuclear Translocation cells are engineered to co-two proteins: a) Enzyme Donor (ED) tagged target protein; b) an Enzyme Acceptor (EA), which is localized to the nucleus. Depending on the assay, activation of the signaling pathway can either a) induce translocation of the ED-tagged target protein into the nucleus, which will force complementation of the two enzyme fragments, and result in the formation of a functional enzyme, that will hydrolyze substrate and generate a chemiluminescent signal; or b) induce the ED-tagged protein to vacate the nucleus, resulting in a decrease of functional enzyme and a subsequent decrease of chemiluminescent signal. Some nuclear translocation assays will also co-an untagged secondary protein involved in the pathway of interest. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.
Size: 100 dp(1 x 96-well)/200 dp(2 x 96-well)/1000 dp(10 x 96-well)
Target Species: Human
Storage: Short term (2 weeks): Store in vapor phase of liquid N2
Kit Components: 1.25 x 10^6 cells per vial in 0.1 mL (kit contains cells, detection reagents and plate(s))

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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