The HSP90β (C-terminal) Inhibitor Screening Assay Kit is designed to measure the inhibition of HSP90β binding to its protein target PPID (also known as Cyclophilin D). With this kit, only three simple steps on a microtiter plate are required. First, a sample containing HSP90β, PPID, and an inhibitor of choice is incubated for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Useful for screening inhibitors of HSP90β and for HSP90β binding assays
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
HSP90β (C-terminal), Biotin-labeled: 100 µg; -80°C PPID, GST-tag: 100 µg; -80°C 3x HSP90 Assay Buffer 2: 4 ml; -20°C 3x HSP90 Detection Buffer: 3 ml; -20°C