Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Other Kits > HTS kinesin ATPase Endpoint Assay Biochem Kit

HTS kinesin ATPase Endpoint Assay Biochem Kit

   Share
"ATPase" Related Products.
Cat.No.:
Kit-0119
Product Overview:
Eukaryotic kinesin motor proteins orchestrate a wide range of kinetic events within a cell. They have been shown to move cargo, such as chromosomes and vesicles, along microtubule tracks. They also play a major role in the organization of cytoskeletal architecture as evidenced in the establishment of the microtubule spindle during mitosis. Kinesins operate by utilizing the energy of ATP hydrolysis to move along their microtubule (MT) substrates. Once a kinesin motor binds to its MT track, the ATPase rate of the motor is often enhanced several hundred to several thousand-fold. MT activated kinesin ATPase is a major parameter in motor function and serves as a powerful method to monitor and study kinesin activity under various experimental conditions.As part of its Cytoskeleton Motor Werks (CMW) line of research reagents, Cytoskeleton, Inc. has developed the Kinesin ATPase End-Point Biochem Kit. The kinesin end-point assay is an extremely quick and economical way to measure inorganic phosphate (Pi) generated during the microtubule activated ATPase activity of kinesin motor proteins. Large numbers of assays can be performed simultaneously in a homogenous reaction, making the assay highly suitable for HTS applications. The assay is based upon a colorimetric change, measured at 650 nm.
Size:
1000 assays
Applications:
1. Measuring microtubule-activated kinesin ATPase activity.2. Discovering/characterizing kinesin inhibitors.3. Discovering/characterizing kinesin regulating cofactors.
Kit Components:
CytoPhos Reagent: One bottle. 70 ml. Phosphate standard: One tube. Contains 1 ml of 0.1 mM phosphate standard. Kinesin Motor Protein: 2 tubes of kinesin motor domain-GST tagged protein, lyophilized. Pre-formed Microtubules: 4 tubes, lyophilized. Kinesin Reaction Buffer: 1 bottle. 100 ml. Taxol: 4 tubes, lyophilized. DMSO: 1 tube, used to resuspend taxol. Labels for Aliquoted Reagents: Microtubules (MTs) need to be aliquoted into 40 individual tubes. The kit box supplied can be used as storage container for these aliquots and the label provided can be used to identify these frozen stocks. See Section VI for details.The label is "BK053 MT STOCKS". A label is also supplied for ATP stocks. The ATP is not provided in this kit.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.