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Hexokinase Inhibitor Screening Kit

Cat.No. : Kit-2144
Product Overview : In the Hexokinase Inhibitor Screening kit, glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. In the presence of a Hexokinase inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of absorbance. This assay kit can be used to screen/study/characterize the potential inhibitors of Hexokinase. The assay is simple, high-throughput adaptable and can be performed within 30 min.
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Size : 100 assays
Applications : Screening/characterizing/studying potential inhibitors of Hexokinase
Storage : Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.HK Assay Buffer: Bring to room temperature before use. Store at -20°C or 4°C.HK Coenzyme and HK Developer: Reconstitute each with 220 µl dH2O. Pipette up and down to dissolve completely. Store at -20°C. Use within two months. Keep on ice while in use.HK Converter: Reconstitute with 220 µl HK Assay Buffer. Aliquot and store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Usewithin two months.Hexokinase: Reconstitute with 160 µl HK Assay Buffer. Aliquot and store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Usewithin two months.HK Inhibitor Control: Reconstitute with 100 µl dH2O. Aliquot and store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Use within two months.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can HK protein be a biomarker for certain diseases? 01/05/2019

Yes, altered levels or activity of HK protein can serve as a biomarker for certain metabolic and neoplastic conditions.

Is there a relationship between HK protein and neurodegenerative diseases? 11/19/2018

Some studies suggest a potential link between altered glucose metabolism, including HK protein dysfunction, and neurodegenerative diseases.

How is HK protein involved in the Warburg effect? 09/01/2017

HK protein plays a role in the Warburg effect, a metabolic shift observed in cancer cells where they preferentially utilize glycolysis even in the presence of oxygen.

How is HK protein involved in diabetes? 10/13/2016

In diabetes, impaired regulation of HK protein can lead to abnormal glucose metabolism and contribute to elevated blood sugar levels.

Can HK protein be targeted for personalized medicine approaches? 02/15/2016

Yes, understanding the individual variations in HK protein expression and activity could contribute to personalized therapeutic strategies.

Customer Reviews (3)

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Reviews
07/29/2022

    The manufacturer understands the importance of customization and can offer tailored solutions to meet specific research needs.

    06/22/2021

      The HK protein's exceptional qualities, combined with the manufacturer's comprehensive technical support, make it an ideal choice for researchers seeking reliable experimental materials and reliable assistance in their scientific endeavors.

      12/09/2017

        From recombinant protein production to modification or labeling services, the company is committed to assisting researchers in obtaining the HK protein in the format and specifications required for their particular studies.

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