Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Detection Kits > Highly Stable ATP Assay Kit
"ATP" Related Products.
Product Overview:
Highly Stable ATP Assay Kit is a high throughput and extremely sensitive detection of ATP production or consumption.
Adenosine-5"-triphosphate (ATP) is a central molecule in the chemistry of all living things and is used to monitor many biological processes. An accurate, reliable method to detect minute ATP levels such as the Luciferase/Luciferin system has broad application. Conventional Luciferase/Luciferin ATP detection systems are unstable since luciferase loses activity rapidly. We have developed a highly stable Luciferase; a genetically modified variant derived from the Luciferase of Diaphanes pectinealis (Chinese Firefly) endemic to Yunnan province, China. We designate our recombinant highly stable luciferase rLucHS. Compared to the normal phenotype of Photinus pyralis, rLucHS provides enhanced stability, excellent sensitivity, and a broader and more physiologically relevant effective pH range. At all pH"s below ~ 8.2, rLucHS has a significantly higher relative activity than Photinus luciferase and is stable for weeks at room temperature and >60 minutes at 37°C. Using the protocol outlined here, the quantitation range is between approximately 1 nmol to 10 fmol/assay. The specific activity of rLucHS is ~ 5 x 1011 RLU/mg protein. The assay can be fully automated for high throughput (1 sec/sample) and is extremely sensitive and is ideal for detecting ATP production or consumption in a variety of processes and enzymatic reactions.
Target Species:
For research use only (RUO)
Store kit at -20°C.
Kit Components:
10X Reaction Buffer. Cap code: purple. 2.0 mLReconstitution Buffer. Cap code: clear. 1.1 mLEnzyme Mix (lyophilized). Cap code: green. 1 vialATP Standard (MW 551) (lyophilized). Cap code: yellow. 1 vial
Detection method:
Compatible Sample Types:
Cell Homogenate, Tissue Homogenate

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.