Human Hexokinase Inhibitor Screening Kit
Cat.No. : | Kit-2111 |
Product Overview : | Human Hexokinase II (HKII) Inhibitor Screening kit uses the HKII"s ability to convert glucose into glucose-6-phosphate. Glucose-6-phosphate is oxidized by glucose-6- phosphate dehydrogenase to form NADH, which reduces a probe that shows strong absorbance at 450 nm. In the presence of Bromopyruvic Acid, reactions are impeded, thus decreasing the rate and/or extent of generation of Hexokinase -dependent absorbance at OD 450 nm. Hexokinase Inhibitor Control is included to compare the efficacy of test inhibitors. This kit provides a sensitive, quick, and easy method for screening potential inhibitors of Hexokinase. The assay is high-throughput adaptable and can be performed in less than 30 min. |
- Specification
- Gene Information
- Related Products
Size : | 100 assays |
Applications : | Screening/characterizing/studying potential inhibitors of human Hexokinase |
Storage : | Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.HK Assay Buffer: Bring to room temperature before use. Store at -20 °C or 4°C.HK Coenzyme and HK Developer: Reconstitute each with 220 µl dH2O. Pipette up and down to dissolve completely. Store at -20°C. Use within two months. Keep on ice while in use.HK Converter: Reconstitute with 220 µl HK Assay Buffer. Aliquot and store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Usewithin two months.Hexokinase II: Ready to use. Store at -20°C.HK Inhibitor Control: Reconstitute with 100 µl dH2O. Aliquot and store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Use within two months. |
Products Types
◆ Assay kits | ||
Kit-1090 | Hexokinase Activity Assay Kit (Fluorometric) | +Inquiry |
Kit-2144 | Hexokinase Inhibitor Screening Kit | +Inquiry |
Kit-0464 | Hexokinase Colorimetric Assay Kit | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, altered levels or activity of HK protein can serve as a biomarker for certain metabolic and neoplastic conditions.
Some studies suggest a potential link between altered glucose metabolism, including HK protein dysfunction, and neurodegenerative diseases.
HK protein plays a role in the Warburg effect, a metabolic shift observed in cancer cells where they preferentially utilize glycolysis even in the presence of oxygen.
In diabetes, impaired regulation of HK protein can lead to abnormal glucose metabolism and contribute to elevated blood sugar levels.
Yes, understanding the individual variations in HK protein expression and activity could contribute to personalized therapeutic strategies.
Customer Reviews (3)
Write a reviewThe manufacturer understands the importance of customization and can offer tailored solutions to meet specific research needs.
The HK protein's exceptional qualities, combined with the manufacturer's comprehensive technical support, make it an ideal choice for researchers seeking reliable experimental materials and reliable assistance in their scientific endeavors.
From recombinant protein production to modification or labeling services, the company is committed to assisting researchers in obtaining the HK protein in the format and specifications required for their particular studies.
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