Indican Assay Kit is used for quantitative determination of indican (indoxyl sulfate) in using colormetric methods.
Indican (indoxyl sulfate) is a putrefaction product resulting from bacterial deconjugation of dietary tryptophan to indole in the small intestine. A large body of evidence supports the use of urinary indican testing as a beneficial point-of-care screening tool for dysbiosis, small intestinal bacterial overgrowth (SIBO), constipation, malabsorption, intestinal mucosal permeability etc. Common microorganisms that contribute to positive indican tests include Salmonella, Shigella, Campylobacter jejuni, yersinia enterocolitica, Citrobacter freundi, Citrobacter diversus, Klebsiella pneumoniae, Pseudomonas aeruginosa, certain strains of Escherichia coli, Staphyloccocus aureus, some strains of bacteriodes, Clostridium difficile, Candida albicans, other candida species etc.
Quantitative direct determination of indican in urine samples.
For research use only (RUO)
Store Reagent A at room temperature and other components at -20°C. Shelf life of 12 months after receipt.
Reagent A 200 mLReagent B 15 mLStandard ( 30 mg/dL) 15 mL*For 96-well plate: 1000 assays*For cuvette: 200 assays
Compatible Sample Types:
Features & Benefits:
Fast and sensitive. Linear detection range: 0.2 - 20 mg/dL (8-800 µmoles/L).Fast and high-throughput. Homogeneous "mix-incubate-measure" type assay. Assay takes only 10 min.Can be readily automated for processing thousands of samples per day.