Invertase Assay Kit is a quantitative determination of invertase/sucrase activity.
INVERTASE (β-fructofuranosidase, EC 188.8.131.52) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertases cleave at the O-C(fructose) bond, whereas a related enzyme sucrose (EC 184.108.40.206) cleaves at the O-C(glucose) bond. A wide range of microorganisms produce invertase and can, thus, utilize sucrose as a nutrient. Invertase assay finds wide applications in environmental (e.g. soil), agricultural and food (confectionery) industry. Invertase Assay Kit provides a convenient and ultra-sensitive colorimetric and fluorimetric means to measure invertase activity. In the assay, invertase cleaves sucrose, resulting in the formation of fructose and glucose, which is determined by a colorimetric (570nm) or fluorimetric method (λem/ex = 585/530nm). The assay is simple, sensitive, stable and high-throughput adaptable.
Invertase and sucrase activity determination in biological and environmental (e.g. soil) samples.Evaluation and screening for invertase inhibitors.
For research use only (RUO)
Store 10x Reaction Buffer and Assay Buffer at 4°C and other reagents at -20°C. Shelf life of six months after receipt.
10x Reaction Buffer: (pH 4.5) 12 mLAssay Buffer 10 mLGlucose Standard 1 mL10x Sucrose 1.5 mLEnzyme Mix 120 uLDye Reagent 120 uL
Compatible Sample Types:
Biological Sample, Environmental (e.g. soil) Sample
Features & Benefits:
Safe: Non-radioactive assay.Sensitive and accurate: As low as 0.007 U/L invertase activity can be quantified.Homogeneous and convenient: "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.