Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Isocitrate Colorimetric Assay Kit

Isocitrate Colorimetric Assay Kit

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Cat.No.:
Kit-0483
Product Overview:
Isocitrate Assay Kit (Colorimetric) is used for quantitative determination of Isocitrate (Isocitric Acid) using colourmetric methods.
Description:
ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.This isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration.
Applications:
Isocitrate determination in food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Usage:
For research use only (RUO)
Storage:
Store all components at -20°C upon receiving.
Kit Components:
Assay Buffer 10 mLEnzyme A 120 µLNADP/MTT 1 mLEnzyme B 120 µLStandard 1 mL
Detection method:
Colorimetric
Compatible Sample Types:
Beverage, Food, Cell lysate, Serum, Tissue homogenate
Features & Benefits:
Fast and sensitive. Linear detection range (2 µL sample): 2 to 5 µM for 1 min reaction.Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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