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JARID Demethylase Activity/Inhibition Colorimetric Assay Kit

Cat.No. : Kit-0485
Product Overview : JARID Demethylase Activity/Inhibition Assay Kit (Colorimetric) is use for measuring activity or inhibition of total JARID.
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Applications : JARID Demethylase Activity/Inhibition Assay Kit (Colorimetric) is suitable for measuring activity or inhibition of total JARID usingnuclear extracts or subtype JARID (JARID1A-JARID1D) purified enzymes from a broad range of species such as mammalians, plant, fungal, and bacterial types, in a variety of forms including cultured cells and fresh tissues. Nuclear extracts can be prepared by using your own successful method. Nuclear extracts can be used immediately or stored at –80°C for future use. Purified enzymes can be active JARIDs from recombinant proteins or isolated from cell/tissues.
Storage : Upon receipt: (1) Store JC3, JC4, and JC6 at –20°C away from light; (2) Store JC1, JC5, JC7, Co-Factor 1, Co-Factor 2, Co-Factor 3, and 8-Well Assay Strips at 4°C away from light; (3) Store remaining components (JC2, JC8, and Adhesive Covering Film) at room temperature away from light.All components of the kit are stable for 6 months from the date of shipment, when stored properly.Note: (1) Check if JC1 (10X Wash Buffer) contains salt precipitates before use. If so, warm (at room temperature or 37°C) and shake the buffer until the salts are re-dissolved; and (2) check if a blue color is present in JC7 (Developer Solution), which indicates contamination of the solution and should not be used. To avoid contamination, transfer the amount of JC7 required into a secondary container (tube or vial) before adding JC7 into the assay wells.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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