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Home > Products > Assay Kits > Cellular Kits > JC-1 Mitochondrial Membrane Potential Assay Kit

JC-1 Mitochondrial Membrane Potential Assay Kit

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Cat.No.:
Kit-0487
Product Overview:
JC-1 Mitochondrial Membrane Potential Assay Kit is a fluorescence-based method for tracing the behavior of mitochondria.
Description:
ΔΨM is an important parameter of mitochondrial function and has been used as an indicator of cell health. Variation of ΔΨM have been previously studied using cationic dyes such as rhodamine-123 (Rh123) and DiOC6. More recently, a cytofluorimetric, lipophilic cationic dye, 5,5",6,6"-tetrachloro-1,1",3,3"-tetraethylbenzimi -dazolylcarbocyanine iodide (JC-1), has been developed. JC-1 has advantages over other cationic dyes in that it enters the mitochondria and changes its fluorescent properties based on the aggregation of the probe. In healthy cells with high ΔΨM, JC-1 forms complexes known as J-aggregates with intense red fluorescence. However, in cells with low ΔΨM, JC-1 remains in the monomeric form, which exhibits green fluorescence. The higher the ratio of red to green fluorescence, the higher the polarization of the mitochondrial membrane.
Usage:
For research use only (RUO)
Storage:
JC-1 Reagent -20°CCell-Based Assay Buffer Tablet Room Temperature
Kit Components:
JC-1 Reagent 500 μLCell-Based Assay Buffer Tablet 3 tablets


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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