JMJD2B is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3.
The JMJD2B Homogeneous Assay Kit is designed to measure activity of the JMJD2B for screening and profiling applications, using AlphaLISA technology. The key to the JMJD2B Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2B enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
JMJD2B (KDM4B), GST-tag: 60 µg; -80°C Primary antibody 5: 2 x 12.5 μl; -80°C Biotinylated histone H3 peptide substrate (JMJD2B): 400 μl; -80°C 4x JMJD2B Assay Buffer: 2 ml; -20°C 4x JMJ assay buffer 2 (Incomplete buffer): 1 ml; -20°C 4x Detection buffer: 2 ml; -20°C