Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Signaling Kits > JNK Activity Screening Kit

JNK Activity Screening Kit

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By Species
Human(1)
By Source
E.coli(1)
By Tag
GST(1)
Cat.No.:
Kit-0491
Product Overview:
JNK Activity Screening Kit is used for detecting JNK activity.
Description:
c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes. The JNK Activity screening kit utilizes an N-terminal c-Jun (1-79) fusion protein bound to glutathion sepharose beads to selectively "pull down" JNK from cell lysates. After wash to remove nonspecifically bound proteins, the kinase reaction is then carried out in the presence of cold ATP. c-Jun phosphorylation is then measured by Western blot analysis using a phospho-c-Jun specific antibody.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store kit at -20°C.
Kit Components:
1. Kinase Extraction Buffer: 80 ml.2. c-Jun (1-79) Fusion Protein Beads: 800 µl.3. Kinase Assay Buffer: 25 ml.4. ATP (10 mM): 50 µl.5. Phospho-cJun Specific Antibody: 50 µl.
Compatible Sample Types:
Cell Lysate, Tissue Lysate


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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