Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > L3MBTL1 TR-FRET Assay Kit
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384 reactions
The L3MBTL1 TR-FRET Assay Kit is designed to measure the inhibition of L3MBTL1 binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, L3MBTL1, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Useful for the study of MBT domain binding assays, screening inhibitors, and selectivity profiling
Stable at least 6 months from date of receipt, when stored as directed
Kit Components:
GST-L3MBTL1: 10 µg; -80°CMethylated MBT Ligand 1: 50 µl; -80°CNon-Methylated MBT Ligand 1: 15 μl; -80°CTb donor: 20 µl; -20°CDye-labeled acceptor: 20 µl; -20°C3x L3MBTL1 TR-FRET Assay Buffer: 4 ml; -20°CNonbinding Corning, low volume, microtiter plate: 1; Room temp.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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