"L3MBTL1" Related Products

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L3MBTL1 TR-FRET Assay Kit

Cat.No.: Kit-1780
Size: 384 reactions
Description: The L3MBTL1 TR-FRET Assay Kit is designed to measure the inhibition of L3MBTL1 binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, L3MBTL1, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Applications: Useful for the study of MBT domain binding assays, screening inhibitors, and selectivity profiling
Storage: Stable at least 6 months from date of receipt, when stored as directed
Kit Components: GST-L3MBTL1: 10 µg; -80°CMethylated MBT Ligand 1: 50 µl; -80°CNon-Methylated MBT Ligand 1: 15 μl; -80°CTb donor: 20 µl; -20°CDye-labeled acceptor: 20 µl; -20°C3x L3MBTL1 TR-FRET Assay Buffer: 4 ml; -20°CNonbinding Corning, low volume, microtiter plate: 1; Room temp.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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