"Lactate" Related Products

Lactate Assay Kit II

Cat.No.: Kit-0499
Product Overview: Lactate Assay Kit II is used for detecting L(+)-Lactate.
Description: Lactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormally high concentrations of lactate have been related to disease states such as diabetes and lactic acidosis, etc. L(+)-Lactate is the major lactate stereoisomer formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate. In the Lactate Assay Kit, lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (λmax = 450 nm). The kit detects L(+)-Lactate in biological samples such as serum or plasma, cells, culture and fermentation media at a level of 0.02 mM – 10 mM. There is no need for pretreatment or purification of samples.
Target Species: Mammals
Usage: For research use only (RUO)
Storage: Store the kit at -20°C.
Kit Components: Lactate Assay Buffer 25 mLLactate Enzyme Mix (Lyophilized) 1 vialLactate Substrate Mix (Lyophilized) 1 vialL(+)‐Lactate Standard (100 mM) 100 μL
Detection method: Colorimetric
Compatible Sample Types: Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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