Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Lactate Dehydrogenase Assay Kit

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Cat.No.:
Kit-0509
Product Overview:
Detection and Quantification of Lactate Dehydrogenase Activity.
Description:
Lactate dehydrogenase (LDH) is an oxidoreductase present in a wide variety of organisms. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD. When disease or injury or toxic material damages tissues, cells release LDH into the bloodstream. Since LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has broad range of applications. In this colorimetric LDH quantification assay, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (Lambda max = 450 nm). The kit quantifies LDH activity in variety of biological samples such as in serum or plasma, cells, culture medium and fermentation, etc. The assay is quick, convenient, and sensitive.
Applications:
Functional Assay
Storage:
Shipped and store at 4°C for 6 months.
Kit Components:
Component-Volume-Storage1. 96-Well Microplate; 2. Extract: 30 ml x 1, 4°C; 3. Reagent I: 5 ml x 1, 4°C; 4. Reagent II: Powder x 1, -20°C; 5. Reagent III: 5 ml x 1, 4°C; 6. Reagent IV: 20 ml x 1, 4°C; 7. Technical Manual: 1 ManualNote: Reagent II: add 1.3 ml distilled water to dissolve before use, stored at -20°C.
Detection method:
Colorimetric
Compatible Sample Types:
Biological Fluid; Cell Culture Supernatant; Plasma; Serum; Tissue Culture Supernatant; Urine


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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