Simple procedure Fast and convenient Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples
Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. BioVision"s Lipid Peroxidation Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (λ = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
The kit detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
For Research Use Only! Not For Use in Humans.
• MDA Lysis Buffer• Phosphotungstic Acid Solution• BHT (100X) • TBA • MDA Standard (4.17M)
Absorbance (532 nm) or Fluorescence (Ex/Em 532/553 nm)
Compatible Sample Types:
Cell and Tissue culture supernatants, plasma and other biological fluids (optimized by end user).