Lipoxygenase Inhibitor Screening Assay Kit detects and measures the hydroperoxides produced in the lipoxygenation reaction using a purified Lipoxygenases (LOs). The detection reaction is equally sensitive to hydroperoxides at various positions within the fatty acid, and will work with fatty acids of any carbon length. It is thus a general detection method for LO, and can be used to screen libraries of compounds for those which inhibit LO enzymes.
Reacts with: HumanPredicted to work with: all Mammals
Kit will arrive packaged as a 4°C kit. For best results, remove components and store as stated below. Item Storage Lipoxygenase Inhibitor Screening Assay Buffer (10X) 4°C Developing Reagent 1 4°C Developing Reagent 2 4°C 15-Lipoxygenase Standard 4°C Arachidonic Acid (substrate) -20°C Linoleic Acid (substrate) -20°C Potassium Hydroxide 4°C 96-Well Solid Plate (Colorimetric Assay) RT 96-Well Cover Sheet RT
Components 96 tests15-Lipoxygenase Standard 1 vial96-Well Plate (Colorimetric Assay) 1 unit96-Well Plate Cover 1 unitArachidonic Acid (substrate) 1 vialDeveloping Reagent 1 1 vialDeveloping Reagent 2 1 vialLinoleic Acid (substrate) 1 vialLipoxygenase Inhibitor Screening Assay Buffer (10X) 1 vialPotassium Hydroxide 1 vial