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Luciferase Reporter Assay Kit

Cat.No.: Kit-0528
Product Overview: This Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells. (200 assays) Simple procedure; takes ~ 20-30 minutesFast and convenientThe assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple samples.
Size: 200 assays
Description: Firefly luciferase has been used as a sensitive reporter for studying gene regulation and function. Luciferase provides a 1000-fold increase in sensitivity in comparison to the standard chloramphenicol acetyltransferase (CAT) assay. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. The light emission from the reaction can be recorded using a luminometer. Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells. For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, Mg2+ and buffer are added to the lysate (Inclusion of CoA yields a nearly constant light emission rather than typical flash kinetics). The luminescent reaction is then triggered by an injection of luciferin, and the emitted light is recorded by a luminometer. By providing CoA, along with ATP, Mg2+ in an optimized buffer solution, Luciferase Reporter Assay Kit ensures maximal sensitivity, consistent light output, as well as convenience and consistency when working with multiple samples.
Applications: This Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells.
Target Species: Mammalian
Usage: This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
Kit Components: Substrate ASubstrate BCell Lysis Buffer
Detection method: Luminescence
Compatible Sample Types: Tissue LysatesCell Lysates

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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