Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > MDA Assay Kit

MDA Assay Kit

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Cat.No.:
Kit-0543
Product Overview:
MDA Assay Kit is a quantitative colormetric/fluorometric determination of lipid peroxidation.
Description:
Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural biproducts. Measuring end products of lipid peroxidation is useful measure of oxidative damage. MDA Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct which can be easily quantified colorimetrically (OD 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
Usage:
For research use only (RUO)
Storage:
Store the kit at -20°C, protected from light. Allow all components to warm to room temperature before use.Briefly centrifuge vials before opening. Read the entire protocol before performing the assay. Store all reagents at -20°C.
Kit Components:
MDA Lysis Buffer. Cap code: WM. 25 mLPhosphotungstic Acid Solution. Cap code: NM. 12.5 mLBHT (100X). Cap code: purple. 1 mLTBA. Cap code: NM. 4 bottlesMDA Standard (4.17M). Cap code: yellow 100 μL
Detection method:
Colorimetric, Fluorometric
Compatible Sample Types:
Biological fluids, Cell Culture Supernatant, Plasma, Tissue Culture Supernatant


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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