|Description:||The MLL1 Complex Direct Activity Chemiluminescent Assay kit is designed to measure MLL1 activity for screening and profiling applications, using purified MLL1 and its complex components: WDR5, RbBP5,Ash2, and DPY30. The key to the MLL1 Complex Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K4 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.|
|Applications:||Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.|
|Storage:||12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.|
|Kit Components:||MLL1/WDR5/Ash2L/RbBP5/DPY30: 25 µg; -80°C 20 µM S-adenosylmethionine: 250 µl; -80°C Primary antibody 2: 12.5 µl; -80°C 4x HMT assay buffer 1: 3 ml; -20°C Secondary HRP-labeled antibody 1: 10 µl; -80°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate (2 components): 6 ml; each +4°C 96-well plate: 1; plate +4°C|
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