Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Other Kits > MLL1 SAM-Screener Assay Kit

MLL1 SAM-Screener Assay Kit

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By Species
Mouse(2)
By Source
By Tag
His(1)
Cat.No.:
Kit-1977
Size:
384 wells
Description:
Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes S-adenosyl-L-methionine (SAM or AdoMet) as a co-factor to catalyze the methylation of the epsilon amino group of lysine. Mixed-lineage leukemia (MLL1) is a member of the trithorax group (trxG)/Set1-like family of gene activators that contains histone methyltransferase activity specific for lysine 4 of histone H3. This methylation plays an important role in gene activation at various developmentally regulated loci, such as the Hox gene loci.This fluorescence polarization assay is based upon a proprietary small molecule fluorescent probe* that binds to the SAM binding pocket in MLL1. Binding of the small molecule probe to MLL1 induces an increase in fluorescence polarization. Binding of the probe can be competed with the endogenous cofactor SAM or by the inhibitor sinefungin, but is unaffected by the histone H3 peptide substrate. The MLL1 SAM-Screener Assay is robust (Z" >0.6) and exhibits a greater than 100 mP shift over a range of 0-500 nM MLL1. The assay is suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1,536-well) if desired.
Storage:
-80°C
Kit Components:
SAM-Binding Site Assay Buffer (10X): 1 vial/2 ml; 1 vial/10 ml; -20°CMLL1 (human recombinant) Assay Enzyme: 1 vial/550 µl; 5 vial/550 µl; -80°CSAM-Binding Site Probe*: 1 vial; 5 vials; -20°CSAM-Binding Site Positive Control: 1 vial/100 µg; 5 vials/100 µg; -20°C384-Well Solid Plate (low volume; black) 1 plate: 5 plates; RT Foil Plate Covers: 1 cover; 5 covers; RT


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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