"MMP" Related Products

MMP-3 Activity Assay Kit

Cat.No.: Kit-0588
Product Overview: The assay sensitivity is< 50 µU. (100 assays) Simple procedure; takes ~ 60 minutes;Fast and convenient;Sensitive assays for measuring MMP-3 in various biological samples.
Size: 100 assays
Description: The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development. In MMP-3 Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em = 325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is< 50 µU.
Applications: The assay sensitivity is< 50 µU.
Target Species: Mammalian
Usage: This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
Kit Components: MMP-3 Assay BufferMMP-3 SubstrateMca Standard (1mM)MMP-3 Positive Control
Detection method: Fluorometer
Compatible Sample Types: Cell Culture SupernatantsPlasmaSerumUrine

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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