Simple procedure; takes ~ 60 minutesFast and convenient Sensitive assays for measuring MMP-3 in various biological samples
The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development. In BioVision"s MMP-3 Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is< 50 µU.
The assay sensitivity is< 50 µU
For Research Use Only! Not For Use in Humans.
• MMP-3 Assay Buffer• MMP-3 Substrate • Mca Standard (1mM) • MMP-3 Positive Control
Fluorescence (Ex/Em 325/393 nm)
Compatible Sample Types:
Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food etc.