"MR" Related Products

MR (human) Reporter Assay Kit

Cat.No.: Kit-1980
Size: 96 wells
Description: This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive high-level expression of full-length, unmodified human mineralocorticoid receptor (NR3C2), a ligand-dependent transcription factor commonly referred to as MR. The reporter cells include the luciferase reporter gene functionally linked to a MR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in MR activity. Luciferase gene expression occurs after ligand-bound MR undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the reporter gene. Unlike some other cell-based assay strategies, the readout from The reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo. MR reporter cells are prepared using the proprietary CryoMite process. This cryopreservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. The nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to MR reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user"s test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate.
Storage: -80°C
Kit Components: ▪ MR Reporter Cells: 3 x 0.60 mL; -80°C ▪ Cell Recovery Medium (CRM): 1 x 10.5 mL; -20°C▪ Compound Screening Medium (CSM): 1 x 35 mL; -20°C▪ Aldosterone, 10 µM (in DMSO) (reference agonist for MR): 1 x 30 µL; -20°C▪ Detection Substrate: 3 x 2.0 mL; -80°C▪ Detection Buffer: 3 x 2.0 mL; -80°C▪ Plate frame: 1; ambient ▪ Snap-in, 8-well strips: 12; ambient

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.