Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Malate Assay Kit

Malate Assay Kit

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Cat.No.:
Kit-0531
Product Overview:
Malate Assay Kit is used to detecting Malate.
Description:
L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others. We has developed an easy and sensitive assay to measure the L(-) Malate level in a variety of samples. In the assay, malate is specifically oxidized to generate a product which reacts with a substrate probe to generate color (λmax = 450 nm). The assay can detect 1 ~ 35 nmol of Malate in a 50 µl sample with a detection sensitivity ~20 µM.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store kit at ‐20°C, protect from light. Warm Malate Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening. Read the entire protocol before use.
Kit Components:
Malate Assay Buffer 20 mlMalate Enzyme Mix: lyophilized. 1 vialWST Substrate: lyophilized. 1 vialMalate Standard (10 µmol): lyophilized. 1 vial
Detection method:
Colorimetric
Compatible Sample Types:
Beer, Biological Fluid, Cell Culture Supernatant, Cheese, Food, Fruit, Plasma, Serum, Tissue Culture Supernatant, Urine, Wine


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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