|Description:||The Melanocortin-3 receptor (MC3R) helps regulate energy homeostasis and mice lacking MC3R have increased fat mass and reduced lean mass. Therefore, agonists that selectively activate MC3R might have beneficial effects related to weight gain and glucose metabolism. This assay consists of a 96-well plate coated with a transfection complex containing DNA constructs for MC3R and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (MC3R Reverse Transfection Strip Plate). Cells grown on the transfection complex will express MC3R at the cell surface. Binding of agonists to MC3R initiates a signal transduction resulting in expression of SEAP. SEAP activity is measured following addition of luminescence-based alkaline phosphatase substrate provided in the kit.|
|Kit Components:||Melanocortin-3 Receptor Reverse Transfection Strip Plate: 1 plate; -20°C Melanocortin Assay α-MSH Positive Control: 1 vial/10 µl; -20°CSEAP Substrate (Luminescence): 1 vial/15 ml; 4°C 96-Well Solid Plate (black) with lid: 3 plates; Room Temperature|
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