Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Signaling Kits > Melanocortin-4 Receptor Reporter Assay Kit

Melanocortin-4 Receptor Reporter Assay Kit

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100 tests
The melanocortin-4 receptor (MC4R) has important roles in weight regulation, sexual function, and inflammation. Mice deficient in MC4R have increased lipid deposition associated with elevated adiposity, while mutations in MC4R in humans are associated with early onset or severe obesity. This assay consists of a 96-well plate coated with a transfection complex containing DNA constructs for MC4R and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (MC4R Reverse Transfection Strip Plate). Cells grown on the transfection complex will express MC4R at the cell surface. Binding of agonists to MC4R initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture media. SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit.
Kit Components:
Melanocortin-4 Receptor Reverse Transfection Strip Plate: 1 plate; -20°CMelanocortin Assay α-MSH Positive Control: 1 vial/10 µl; -20°CSEAP Substrate (Luminescence): 1 vial/15 ml; 4°C96-Well Solid Plate (black) with lid: 3 plates; Room Temperature

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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