The melanocortin-4 receptor (MC4R) has important roles in weight regulation, sexual function, and inflammation. Mice deficient in MC4R have increased lipid deposition associated with elevated adiposity, while mutations in MC4R in humans are associated with early onset or severe obesity. This assay consists of a 96-well plate coated with a transfection complex containing DNA constructs for MC4R and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (MC4R Reverse Transfection Strip Plate). Cells grown on the transfection complex will express MC4R at the cell surface. Binding of agonists to MC4R initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture media. SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit.
Melanocortin-4 Receptor Reverse Transfection Strip Plate: 1 plate; -20°CMelanocortin Assay α-MSH Positive Control: 1 vial/10 µl; -20°CSEAP Substrate (Luminescence): 1 vial/15 ml; 4°C96-Well Solid Plate (black) with lid: 3 plates; Room Temperature