Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Mitochondria/Cytosol Fractionation Kit

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Cat.No.:
Kit-0557
Product Overview:
Mitochondria/Cytosol Fractionation Kit is used for isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and non-apoptotic cells.
Description:
The Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
After opening the kit, store buffers at 4°C. Store Protease Inhibitor Cocktail and DTT at -20°C.
Kit Components:
Mitochondria Extraction Buffer: 10 ml5X Cytosol Extraction Buffer: 20 mlDTT (1 M): 110 µlProtease Inhibitor Cocktail* 1 vial*Add 250µl of DMSO, and mix well before use
Compatible Sample Types:
Cell, Tissue


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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