Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Metabolism Kits > Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit

Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit

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Product Overview:
The kit is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample. The enzyme is captured within the wells of the microplate and activity is determined by following the production of NADH in the following ALDH2 catalyzed reaction:acetaldehyde + NAD+ ->acid + NADHThe generation of NADH is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient-37000 M-1cm-1). ab115348 immunocaptures in each well only native ALDH2 from the chosen sample; this removes all other enzymes, including unrelated aldehyde dehydrogenases.
1 x 96 test
Functional Studies
Target Species:
Reacts with: Mouse, Rat, Cow, Dog, Human
All components are shipped cold. Reagent dye, Coupler and NAD+ are shipped lyophilized. Before use rehydrate by adding 0.25 mL pure H2O to each tube and vortex each tube thoroughly to dissolve. After hydration unused amounts of these three materials should be stored at -80°C. Store all other components store at 4°C. This kit is Stable for 6 months from receipt.
Kit Components:
Components 1 x 96 tests100X acetaldehyde (2.5 M) 1 x 0.25ml100X Coupler 1 x 0.25ml100X NAD+ 1 x 0.25ml100X Reagent Dye 1 x 0.25ml10X Blocking Buffer 1 x 6ml1X Base Buffer 1 x 24ml20X Buffer 1 x 20ml96 well microplate (12 strips) 1 unitExtraction Buffer 1 x 15ml
Detection method:
Compatible Sample Types:
Cell culture extracts, Tissue Extracts

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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