NAD (P)H dehydrogenase [quinone] 1 (NQO1) Activity Assay kit is designed for the sensitive and accurate measurement of NQO1 activity in a number of sample types.NQO1 serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways, NQO1 protects against quinone-induced damage by competing with potentially toxic one-electron pathways. It also functions in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.The enzyme activity is determined by following the reduction of Menadione with cofactor NADH and the simultaneous reduction of WST1 which leads to increased absorbance at 440 nm.
Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR" + electron donor (2 e-) + 2H+ → ROH + R"OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.
Reacts with: Mouse, Rat, Cow, Human
Store kit at 4ºC immediately upon receipt.Refer to list of materials supplied for storage conditions of individual components. Observe the storage conditions for individual prepared components in sections 9&10.
Components 1 x 96 tests1000X Cofactor 1 vial1000X Inhibitor 1 vial100X Dye (lyophilized) 1 vial100X Menadione 1 vial100X NADH 1 vial20X Basic Buffer 1 x 3ml2X Extraction Buffer 1 x 15ml96-well microplate 2 units
Compatible Sample Types:
Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts, Cell Lysate, Tissue Homogenate, Tissue Lysate