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Native B. bronchiseptica Dermonecrotic Toxin

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Cat.No.:
dnt-001B
Product Overview:
DNT was purified from B. bronchiseptica strain S798 by propriety chromatography and differential precipitation method.
Description:
Dermonecrotic toxin (DNT) is a common toxin produced by pathogenic bacteria belonging to the genus Bordetella, such as B. pertussis, B. parapertussis, and B. bronchiseptica. This toxin activates small GTP-binding proteins such as Rho, Rac and Cdc42 through deamidation or polyamination for downstream signaling pathways.
Source:
B. bronchiseptica
Species:
B. bronchiseptica
Form:
0.53 mg/ml of DNT in 50 mM sodium phosphate buffer (pH 7.2), 1 M urea, 0.3 M sodium sulfate, filter-sterilized.
Bio-activity:
DNT at 5 ng/ml induces ballooning morphological change in cultured mouse osteoblastic cell line MC3T3-E1.
Purity:
More than 95 % purity by SDS-PAGE (CBB staining)
Applications:
1) For the studies of the signaling pathways mediated by small GTP-binding proteins.
2) Antigen to be used for detection of infection of Bordetella by immunological method.
Notes:
The toxin can be inactivated by boiling for 30 min.
Storage:
Ship and store at 4centigrade.
Warning:
This product is only for research use, not for human use. It should be handled carefully by persons with expertise in knowledge and techniques for the safe handling of bacterial toxins. Avoid mouth pipetting. Wear protective gloves on handling the toxin. Avoid contact with open wounds. Wash thoroughly any area of the body that makes contact with the toxin.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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