Pertussis is lyophilized in pure water, it is a genetically inactivated form of pertussis toxin. This protein may be used as antigens and as carrier proteins for other antigens.
Pertussis toxin (PT), a virulence factor produced by Bordetella pertussis, is a multi-subunit toxin which binds to most cultured mammalian cells and targets specific G protein, inhibiting the ability of the G protein to function in signaling pathways. Depending on the function of the G protein, the effects of PT can vary. This ability to inhibit pathways utilizing the Gi family of protein-coupled receptors is the basis of the use of PT as a tool in cell biology. PT plays a role in infection by suppressing and modulating various host immune responses to Bordetella pertussis. PT has been used to stimulate experimental autoimmune diseases in rodent models, such as experimental auto immune encephalitis (EAE).
Each vial contains 50 ug of protein. Reconstituted with water or buffer of choice. Mix gently prior to use ensure a uniform suspension. Do not sterile filter, as this will result in loss of material. Handle the product gently, do not vortex.
This product was packaged aseptically, and sealed under vacum. Store at 2-8 centigrade prior to and reconstitution reconstitution. Do not freeze.
Good laboratory technique should be employed in the safe handling of this product. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses. Nitrile gloves are recommended when handling lyophilized material. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent.