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Native B. pertussis Pertussis toxin

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Product Overview:
Pertussis toxin is a protein isolated from the Gramnegative coccobaccillus, Bordatella pertussis. The toxin is released in an inactive form. This product consists of 5 subunits.
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.
B. pertussis
B. pertussis
Lyophilized powder containing sodium chloride and sodium phosphate buffer salts.
Molecular Mass:
Molecular mass: ~117 kDa (ultracentrifugation)
Pertussis toxin consists of 5 subunits combined in a 1:1:1:2:1 ratio, with the following molecular masses: S1 = 26,017 Da; S2 = 21,839 Da; S3 = 21,751 Da; S4 = 12,061 Da; S5 = 11,747 Da.
The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.
Store at 2-8 centigrade
When reconstituted with 500 ml of water each vial will contain 0.05 M sodium phosphate, pH 7.2, with 0.5 M sodium chloride.
This pertussis toxin product has not been preactivated with ATP. Preincubate pertussis toxin in the presence of 1-5 mM ATP and 1–5 mM DTT for in vitro use with cell membranes. Preincubation with ATP and DTT is not recommended for use with intact cells or in vivo use.
Islet Activating Protein; Pertussigen; Leukocytosis-Promoting Factor; Lymphocytosis Promoting Factor; Histamine-Sensitizing Factor; Pertussis Exotoxin; PTX; PT

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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