|Product Overview:||Pertussis toxin is a protein isolated from the Gramnegative coccobaccillus, Bordatella pertussis. The toxin is released in an inactive form. This product consists of 5 subunits.|
|Description:||Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.|
|Form:||Aseptically filled solution in 50% glycerol, containing 50 mM TRIS, 10 mM glycine, 0.5 M NaCl, pH 7.5|
|Molecular Mass:||Molecular mass: ~117 kDa (ultracentrifugation)
Pertussis toxin consists of 5 subunits combined in a 1:1:1:2:1 ratio, with the following molecular masses: S1 = 26,017 Da; S2 = 21,839 Da; S3 = 21,751 Da; S4 = 12,061 Da; S5 = 11,747 Da.
|Applications:||The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.|
|Storage:||Store at -20 centigrade|
|Handling:||This pertussis toxin product has not been preactivated with ATP. Preincubate pertussis toxin in the presence of 1-5 mM ATP and 1-5 mM DTT for in vitro use with cell membranes. Preincubation with ATP and DTT is not recommended for use with intact cells or in vivo use.|
|Synonyms:||Islet Activating Protein; Pertussigen; Leukocytosis-Promoting Factor; Lymphocytosis Promoting Factor; Histamine-Sensitizing Factor; Pertussis Exotoxin; PTX; PT|
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