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Home > Products > Toxin > Native B. pertussis Pertussis toxin

Native B. pertussis Pertussis toxin

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Cat.No.:
PTX-108B
Product Overview:
Pertussis toxin is a protein isolated from the Gramnegative coccobaccillus, Bordatella pertussis. The toxin is released in an inactive form. This product consists of 5 subunits.
Description:
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.
Source:
B. pertussis
Species:
B. pertussis
Form:
Aseptically filled solution in 50% glycerol, containing 50 mM TRIS, 10 mM glycine, 0.5 M NaCl, pH 7.5
Molecular Mass:
Molecular mass: ~117 kDa (ultracentrifugation)
Pertussis toxin consists of 5 subunits combined in a 1:1:1:2:1 ratio, with the following molecular masses: S1 = 26,017 Da; S2 = 21,839 Da; S3 = 21,751 Da; S4 = 12,061 Da; S5 = 11,747 Da.
Applications:
The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.
Storage:
Store at -20 centigrade
Handling:
This pertussis toxin product has not been preactivated with ATP. Preincubate pertussis toxin in the presence of 1-5 mM ATP and 1-5 mM DTT for in vitro use with cell membranes. Preincubation with ATP and DTT is not recommended for use with intact cells or in vivo use.
Synonyms:
Islet Activating Protein; Pertussigen; Leukocytosis-Promoting Factor; Lymphocytosis Promoting Factor; Histamine-Sensitizing Factor; Pertussis Exotoxin; PTX; PT


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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