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Native Cholera Toxin (AZIDE-FREE) protein

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Product Overview:
Cholera Toxin (AZIDE-FREE) protein was purified from Vibrio cholerae.
Produced by Vibrio cholerae, cholera toxin (CT) is a multimeric enterotoxin that transfers ADP-ribose to a G protein, locking adenylate cyclase in an 'on' position. By binding to the membrane of enteric cells, cholera toxin stimulates the cellular adenylate cyclase system, causing the hyper-secretion of chloride and bicarbonate ions, which results in increased fluld secretion and the severe diarrhea characteristic of the disease cholera. The pentameric B subunit of CT binds with high efficiency to GM1 monosialoganglioside cell membrane receptors, present in many cell types, allowing its use experimentally in cell culture. Following binding, the toxin is endocytosed and travels retrogradely to the endoplasmic reticulum where the enzymatically active A subunit (CTA1) is translocated to the cytosol. Within the cytosol, CTA1 catalyzes the ADP-ribosylation of the Gs protein, which activates adenylate cyclase and as a consequence increasing the intracellular concentration of cAMP. Relevant to the development of vaccine adjuvants, CT effectively modulates mammalian immune systems and acts as an adjuvant for co-administered antigens.
V. cholerae
V. cholerae
When reconsitituted with 500 ul of water, the buffer is 0.05 M Tris, 0.2 M Nacl, 0.001M Na2EDTA at pH 7.5. Handle the product gently, do not vortex.
The endotoxin content, determined using a kinetic chromagenic LAL assay, is 5 EU/mg.
This product is supplied as a lyophilized powder which has been sealed under vacuum. Store lyophilized vials at 2-8 centigrade. Do not freeze.
1 mg/mL
Good laboratory technique should be employed in the safe handling of this product. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses. Nitrile gloves are recommended when handling lyophilized material. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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