LPS was purified from Escherichia coli J5 (Rc).
Lipopolysaccharides (LPS), also known as lipoglycans and endotoxins, are large molecules consisting of a lipid and a polysaccharide composed of O-antigen, outer core and inner core joined by a covalent bond; they are found in the outer membrane of Gram-negative bacteria, and elicit strong immune responses in animals.
LPS is dispersible in aqueous solvents at concentrations of 1 mg/ml. To achieve suspension in water, heating to about 50 centigrade with intermittent vortexing or sonication is recommedded. Allow ample tion for dispersion to occur. The use of 0.5% triethlamine aids in dispersion. Triehylamine is very basic and may be neutralized with Tris HCl to avoid hydrolysis of the LPS fatty acid chains.
Store at 4 centigrade prior to and following reconstitution.
Good laboratory technique should be employed in the safe handling of this product. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses. Nitrile gloves are recommended when handling lyophilized material. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent.