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Native S. pyogenes Streptolysin O

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Cat.No.:
SLO-176S
Product Overview:
Streptolysin O (SLO) is a single polypeptide chain that is not glycosylated. This product is isolated from Streptococcus pyogenes.
Description:
Streptolysin O possesses a single polypeptide chain with a molecular weight of f62 kDa. Streptolysin O binds to membrane cholesterol and oligomerizes to create a ring structure that consists of 45 to 50 units. The ring structure inserts into the membrane to make a large pore (25 to 30 nm), which DNA, RNA, peptides and proteins may pass. It is thiol-activated. It is inhibited by allicin, an active component of garlic.
Source:
Streptococcus pyogenes
Species:
Streptococcus pyogenes
Form:
Lyophilized powder containing Tris buffer salts, sodium azide, PMSF, and EDTA.
Bio-activity:
The protein content is 3-9% and each vial contains less than 1 mg solid (25,000–50,000 units/vial).
Molecular Mass:
native 69 kDa
Unit Definition:
One unit will cause 50% lysis of 50 ml of a 2% human red blood cell suspension in phosphate buffered saline, pH 7.4, at 37 centigrade for 30 minutes.
Applications:
Permeabilizes membranes to permit cellular uptake of large or charged molecules.
Storage:
Store at 2-8 centigrade
Synonyms:
SLO; Streptolysin O


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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