This toxin was highly purified from growth medium of Vibrio cholerae, 569B strain.
Cholera toxin, a main enterotoxin, interacts with G proteins and increases cyclic AMP in the intestinal lining to open ion channels. As ions flow into the intestinal lumen (lining), body fluids (mostly water) flows out of the body due to osmosis leading to massive diarrhea as the fluid is expelled from the body. Cholera toxin is a complex consisting of one molecule of A subunit (27.2 kD) and 5 molecules of B subunits (11.6 kD). It adsorbs to GM1 ganglioside on the surface of target cells by the B subunit and penetrates into cells where A subunit is dissociated and processed into A1, which constitutively activates adenyl cyclase activity of α subunit of Gs (a kind of GTP-binding protein).
1.0 mg/ml in 50 mM Tris-HCl (pH7.5) 0.2 M NaCl 1mM Na2EDTA, 10% Glycerol
Addition of cholera toxin at ~ 1 ng/ml to the culture medium changed more than 50% of Vero cells into spindle shape.
Cholera toxin is a complex consisting of one molecule of A subunit (27.2 kD) and 5 molecules of B subunits (11.6 kD).
More than 90% purity
1) Detection of GTP-binding protein Gsα
2) Detection of a low molecular weight GTP-binding protein, ARF
3) Manipulation of culture cells to increase cellular concentration of cyclic AMP
4) Growth supplement for many animal cells including iPS cells, tumor , NIH3T3 and MCF10A cells
Ship with dry-ice. Store at -80centigrade.
It should be handled carefully by persons with expertise in knowledge and techniques for the safe handling of Cholera toxin. Avoid mouth pipetting. Wear protective gloves when handling the toxin. Avoid contact with open wounds. Wash thoroughly any area of the body that makes contact with the toxin. It is recommended that persons who handle the toxin are immunized by diphtheria vaccine.