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Home > Products > Toxin > Native V. cholerae Cholera Toxin A Subunit

Native V. cholerae Cholera Toxin A Subunit

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Product Overview:
This product is the cholera toxin A subunit purified from Vibrio cholerae.
Cholera toxin is the virulent factor from Vibrio cholerae that leads to severe diarrhea followed by dehydration in humans. Several bacterial toxins are ADP-ribosyltransferases with protein substrates. Many of the substrates ADP-ribosylated by bacterial protein toxins are G-proteins, which are involved in signal transduction and ADP-ribosylation is one of the more significant post translational modifications of proteins. The ADP-ribosylation activity of cholera toxin activates adenylate cyclase, resulting in the production of cyclic AMP by adenylate cyclase, which causes many metabolic alterations.
V. cholerae
V. cholerae
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA.
ADP-ribosylation activity is measured by ADPribosylation of synthetic poly-L-arginine, following the incorporation of ADP-ribose from [U-14C] adenine NAD to TCA precipitable material. One mg of cholera toxin A subunit causes the incorporation of at least 2 pmole of ADP-ribose in 30 minutes at 30 centigrade
Molecular Mass:
27.2 kDa
≤0.5% Cholera toxin B subunit (SDS-PAGE)
Store at 2-8 centigrade
When reconstituted with 1 mL of water, the solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.
CTA; Cholera Toxin A Subunit; Cholera Toxin

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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