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Home > Products > Toxin > Native V. cholerae Cholera Toxin B subunit

Native V. cholerae Cholera Toxin B subunit

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His(1)
Cat.No.:
CTB-101C
Product Overview:
Cholera Toxin is a heterohexameric AB5 enterotoxin. Cholera Toxin B subunit is the the B subunit pentamer of cholera toxin produced by Vibrio cholera. It is generally involved in the cell trafficking and signalling machineries.
Description:
Cholera toxin belongs to the AB5-subunit family of toxins. The native hexameric protein has a molecular mass of ~85 kDa and contains two subunits. It consists of a single A subunit (~27.2 kDa), responsible for the ADP-ribosylation activity, and five B subunits (~11.6 kDa each), which are arranged as a pentameric ring with an apparent 5-fold symmetry and are associated with the cell surface receptor binding and subsequent internalization (transmembrane transport) of the enzymatic component.
Source:
V. cholerae
Species:
V. cholerae
Form:
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.
Bio-activity:
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.
Molecular Mass:
12 kDa
Purity:
≥95% (SDS-PAGE),≤0.5% Cholera toxin A subunit (SDS-PAGE)
Applications:
Cholera Toxin B subunit has been used for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4. It can also be used in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.
Storage:
Store at 2-8 centigrade
Reconstitution:
When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3 and 1 mM sodium EDTA.
Synonyms:
CTB; Cholera Toxin B subunit


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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