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Native V. cholerae Cholera Toxin B subunit

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Cholera Toxin is a heterohexameric AB5 enterotoxin. Cholera Toxin B subunit is the the B subunit pentamer of cholera toxin produced by Vibrio cholera. It is generally involved in the cell trafficking and signalling machineries.
Cholera toxin belongs to the AB5-subunit family of toxins. The native hexameric protein has a molecular mass of ~85 kDa and contains two subunits. It consists of a single A subunit (~27.2 kDa), responsible for the ADP-ribosylation activity, and five B subunits (~11.6 kDa each), which are arranged as a pentameric ring with an apparent 5-fold symmetry and are associated with the cell surface receptor binding and subsequent internalization (transmembrane transport) of the enzymatic component.
V. cholerae
V. cholerae
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.
Molecular Mass:
12 kDa
≥95% (SDS-PAGE),≤0.5% Cholera toxin A subunit (SDS-PAGE)
Cholera Toxin B subunit has been used for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4. It can also be used in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.
Store at 2-8 centigrade
When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3 and 1 mM sodium EDTA.
CTB; Cholera Toxin B subunit

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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