Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Toxin > Native V. cholerae Cholera Toxin B subunit, FITC labeled

Native V. cholerae Cholera Toxin B subunit, FITC labeled

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His(1)
Cat.No.:
CTB-104C
Product Overview:
This product is the cholera toxin B subunit labeled with fluorescein isothiocyanate (FITC, 389.4 Da). The extent of labeling is ~1 mole of FITC per mole of CTB.
Description:
Cholera toxin belongs to the AB5-subunit family of toxins. The native hexameric protein has a molecular mass of ~85 kDa and contains two subunits. It consists of a single A subunit (~27.2 kDa), responsible for the ADP-ribosylation activity, and five B subunits (~11.6 kDa each), which are arranged as a pentameric ring with an apparent 5-fold symmetry and are associated with the cell surface receptor binding and subsequent internalization (transmembrane transport) of the enzymatic component.
Source:
V. cholerae
Species:
V. cholerae
Form:
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide.
Bio-activity:
Activity measured by ELISA using ganglioside GM1 coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.
Molecular Mass:
12 kDa
Stability:
This product is stable 2 years when stored properly.
Storage:
Store at 2-8 centigrade
Handling:
Cholera toxin is soluble in water at a concentration of 10 mg/ml. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that may lead to foaming. Solutions can be filtered through a 0.2 mm filter.
Synonyms:
CTB; Cholera Toxin B subunit


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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