Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email: info@creative-biomart.com
Home > Products > Toxin > Native V. cholerae Cholera Toxin B subunit, biotin labeled

Native V. cholerae Cholera Toxin B subunit, biotin labeled

   Share
"Cholera Toxin B subunit" Related Products.
By Species
By Source
By Tag
His(1)
Cat.No.:
CTB-103C
Product Overview:
This product is the cholera toxin B subunit (CTB) labeled with biotin (244.3 Da). The extent of labeling is ~1 mole of biotin per mole of CTB. The conjugate is prepared using (+)-biotin N-hydroxysuccinimide ester. The biotin is conjugated to the lysine residues present in the cholera toxin B subunit. It contains ~2% unconjugated biotin.
Description:
Cholera toxin belongs to the AB5-subunit family of toxins. The native hexameric protein has a molecular mass of ~85 kDa and contains two subunits. It consists of a single A subunit (~27.2 kDa), responsible for the ADP-ribosylation activity, and five B subunits (~11.6 kDa each), which are arranged as a pentameric ring with an apparent 5-fold symmetry and are associated with the cell surface receptor binding and subsequent internalization (transmembrane transport) of the enzymatic component.
Source:
V. cholerae
Species:
V. cholerae
Form:
Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA.
Bio-activity:
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared.
Molecular Mass:
12 kDa
Storage:
Store at 2-8 centigrade
Handling:
Cholera toxin is soluble in water at a concentration of 10 mg/ml. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that may lead to foaming. Solutions can be filtered through a 0.2 mm filter.
Synonyms:
CTB; Cholera Toxin B subunit


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.