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Home > Products > Toxin > Native V. cholerae Cholera Toxin B subunit, biotin labeled

Native V. cholerae Cholera Toxin B subunit, biotin labeled

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Product Overview:
This product is the cholera toxin B subunit (CTB) labeled with biotin (244.3 Da). The extent of labeling is ~1 mole of biotin per mole of CTB. The conjugate is prepared using (+)-biotin N-hydroxysuccinimide ester. The biotin is conjugated to the lysine residues present in the cholera toxin B subunit. It contains ~2% unconjugated biotin.
Cholera toxin belongs to the AB5-subunit family of toxins. The native hexameric protein has a molecular mass of ~85 kDa and contains two subunits. It consists of a single A subunit (~27.2 kDa), responsible for the ADP-ribosylation activity, and five B subunits (~11.6 kDa each), which are arranged as a pentameric ring with an apparent 5-fold symmetry and are associated with the cell surface receptor binding and subsequent internalization (transmembrane transport) of the enzymatic component.
V. cholerae
V. cholerae
Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA.
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared.
Molecular Mass:
12 kDa
Store at 2-8 centigrade
Cholera toxin is soluble in water at a concentration of 10 mg/ml. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that may lead to foaming. Solutions can be filtered through a 0.2 mm filter.
CTB; Cholera Toxin B subunit

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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