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Neutrophil elastase colorimetric drug discovery Kit

Cat.No.: Kit-0323
Product Overview: It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays.
Size: 96 wells
Description: Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.
Applications: Colorimetric detection, HTS
Storage: -80°C
Kit Components: Neutrophil elastase (human), (purified)pNA substrate (MeOSuc-AAPV-pNA) (60μL; 20mM in DMSO)pNA calibration standard (p-nitroaniline) (1ml; 50μM in assay buffer)Elastatinal (inhibitor) (10μL; 20mM in DMSO)Neutrophil elastase assay buffer (20ml; 100mM HEPES, pH 7.25, 500mM NaCl, 0.05% Tween-20; liquid in a screw-cap plastic bottle)96-well microplate

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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