Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email:
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Neutrophil elastase colorimetric drug discovery Kit

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Product Overview:
It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays.
96 wells
Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.
Colorimetric detection, HTS
Kit Components:
Neutrophil elastase (human), (purified)pNA substrate (MeOSuc-AAPV-pNA) (60μL; 20mM in DMSO)pNA calibration standard (p-nitroaniline) (1ml; 50μM in assay buffer)Elastatinal (inhibitor) (10μL; 20mM in DMSO)Neutrophil elastase assay buffer (20ml; 100mM HEPES, pH 7.25, 500mM NaCl, 0.05% Tween-20; liquid in a screw-cap plastic bottle)96-well microplate

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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