Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Neutrophil (mouse) Isolation Kit

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Cat.No.:
Kit-1984
Size:
1 ea
Description:
Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive antimicrobial proteins and peptides including lysozyme, myeloperoxidase, neutrophil elastase, cathepsins, and the alpha- and beta-defensins. Neutrophils also form extracellular traps, web-like structures composed of DNA, histones, and various antimicrobial proteins that are extruded from the neutrophil in order to ensnare and kill extracellular pathogens. In mice, neutrophils comprise ~10% of circulating white blood cells, so unlike humans, it is usually not possible to isolate even one million neutrophils from a single mouse from peripheral blood. The Neutrophil (mouse) Isolation Kit provides a streamlined method for isolating mouse neutrophils by peritoneal lavage. Included in this kit are all the reagents necessary for inducing a sterile inflammatory response in the peritoneal cavities of up to five mice, the density separation medium required to separate the neutrophils from other peritoneal leukocytes, and the fluorochrome-labeled antibodies required to assess the success of the isolation procedure by flow cytometry.
Storage:
-20°C
Kit Components:
Sodium Caseinate Solution Assay Reagent (7.5%): 1 vial/5 ml; -20°C Percoll Gradient Assay Reagent (63%): 5 vials/5 ml; Room Temperature RBC Lysis Buffer (10X): 1 vial/10 ml; Room Temperature Bovine Serum Albumin Assay Reagent: 1 vial/5 g; 4°C Anti-Mouse Ly6G PE/CD11b FITC Reagent: 1 vial/1 ml; 4°C


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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