Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Nickel Assay Kit

Nickel Assay Kit

   Share
"Nickel" Related Products.
Cat.No.:
Kit-0624
Product Overview:
Nickel Assay Kit is used for quantifying Ni+2.
Description:
Nickel is one of four ferromagnetic elements (symbol Ni, at. Num. 28). Several enzymes depend on nickel for activity, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO2 to CH4) and some hydrogenases which allow the production or removal of H2. Most of these activities are found in the archaebacteria. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions. The Nickel Assay Kit provides a simple method of quantifying Ni+2 in a variety of samples. The assay takes advantage of reaction of Ni+2 with mercaptoethanol in borate buffer to form a complex with strong absorbance bands from ~300 to 600 nm. Fe+2 and Co+2 interfere with the assay, therefore extra steps (as described below) must be taken to subtract the interference in order to determine the correct Nickel concentration in mixed samples. Other ions tested (Mn+2, Cu+2, Zn+2) do not interfere with the assay, presumably no other ionic species are present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni+2 in a variety of samples, which gives a linear range of 2 to 50 nmol Nickel containing less than 25 nmol Cobalt.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store the kit at room temperature.
Kit Components:
1. Nickel Assay Buffer: 20 ml.2. Nickel Reagent: 1 ml.3. Nickel Chloride Standard (1.0 µmol): lyophilized.
Detection method:
Colorimetric
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.