Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Nitrate/Nitrite Colorimetric Assay Kit (LDH method)

Nitrate/Nitrite Colorimetric Assay Kit (LDH method)

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Cat.No.:
Kit-0628
Product Overview:
Nitrate/Nitrite Colorimetric Assay Kit (LDH method) is a measurement for detecting low levels of nitrite.
Description:
Nitric Oxide (NO) is synthesized in biological systems by the enzyme Nitric Oxide Synthase (NOS). NOS is a remarkably complex enzyme which acts on molecular oxygen, arginine, and NADPH to produce NO, citrulline, and NADP+. This process requires five additional cofactors (FMN, FAD, Heme, calmodulin, and tetrahydrobiopterin) and two divalent cations (calcium and heme iron). Three distinct isoforms of NOS have been identified.
Usage:
For research use only (RUO)
Storage:
This kit will perform as specified if stored at -20°C and used before the expiration date indicated on the outside of the box.Component StorageNitrate/Nitrite Assay Buffer 4°CNitrate Reductase (LDH method) -20°CLactate Dehydrogenase Cofactor Preparation -20°CNitrate Standard 4°C or Room TemperatureLactate Dehydrogenase -20°CGriess Reagent R1 4°CGriess Reagent R2 4°C96-Well Plate (Colorimetric Assay) Room Temperature96-Well Cover Sheets Room Temperature
Kit Components:
Nitrate/Nitrite Assay Buffer 1 vialNitrate Reductase (LDH method) 1 vialLactate Dehydrogenase Cofactor Preparation 1 vialNitrate Standard 1 vialLactate Dehydrogenase 1 vialGriess Reagent R1 1 vialGriess Reagent R2 1 vial96-Well Plate (Colorimetric Assay) 1 plate96-Well Cover Sheets 1 cover


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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